TEHRAN, Young Journalists Club (YJC) - Rouhani's remarks in Tehran Sunday before leaving for New York to attend the UN General Assembly came a day after terrorists attacked a military parade in Ahvaz in southwest Iran, killing 25 people.
"Americans want Iran to have no security. They want to create chaos and turmoil and set the conditions so that they can return to the country one day and take charge as they did in the old days, but none of these is possible," the president said.
"These are unattainable dreams and America will never achieve any of these goals," Rouhani added.
He touched on Washington's hostile policies toward Iran, including its withdrawal from an international nuclear deal with Tehran under "delusional excuses," putting a nation under strain.
"Our people will resist and the government has prepared itself for this confrontation," he said, adding that everyone will "join hands to put these difficult times behind us with the grace of God."
"The Americans will regret what they did because they made a very wrong choice", the president added.
Rouhani also said the US is provoking regional states against Iran, citing the terrorist attack in Ahvaz, which also left scores of people, including women and children, injured.
"It is entirely clear to us who did it, who they are and where they are affiliated to," he said.
"The sponsor of all these small mercenary countries in the region is America. Americans are provoking them and providing the right conditions for them to commit these crimes," Rouhani added.
The president said these acts will have no effect on the will and direction of the Iranian nation which "has stood up and resisted much bigger crimes."
Terrorists opened fire on people watching a military parade held to mark the invasion of Iran by former Iraqi dictator Saddam Hussein during the 1980s.
The Saudi-backed al-Ahvaziya terror group, which is based in Europe, has claimed responsibility for the assault.
Rouhani said as long as Saddam was alive, the group used to be a mercenary of the former Ba'ath regime, but they switched their fealty to a "southern Persian Gulf state" which has undertaken to provide the terrorists with funds, weapons and political assistance.
Members of the terror group, he said, had lived inside Iraq for years but Iran granted them amnesty after the end of the Iraqi-imposed war in 1988. Nevertheless, "they have never regretted or stopped their crimes," he added.
Rouhani described the Saturday attack, especially in a city which "valiantly withstood eight years" of attacks by Saddam's forces, a "big crime" which will not go unpunished.
"Iran's answer to these crimes will be within the framework of law and the country's national interests," he said as he paid tribute to the residents of Ahvaz.
"In the early months of the war, there were days when cannonballs would smash into the streets of Ahvaz and I saw them, but the residents did not evacuate the city and stayed put and resisted," he recounted.
Elsewhere in his remarks, Rouhani hit out at the US for abusing the UN which Trump reportedly plans to use for a series of speeches to push a hard line against Iran.
A year after he shocked his counterparts by threatening to "totally destroy" North Korea in his inaugural UN address, Trump is expected to outline his confrontation with Iran, global trade and his "America first" view of foreign policy.
Trump lawyer calls for 'revolution' in Iran
Speaking in New York on Saturday, his personal lawyer Rudy Giuliani claimed that US sanctions were working in Iran and that the coercive measures could lead to a “successful revolution.”
“I don’t know when we’re going to overthrow them,” he told a meeting of Mujahedin Khalq Organization (MKO) terrorist group. “It could be in a few days, months, a couple of years. But it’s going to happen."
Giuliani's comments contradicted the Trump administration's pronouncements that it was not seeking a government change in Iran.
Washington has long been supporting the MKO. In 2012, the US State Department removed the group from its list of designated terrorist organizations.
Nevertheless, the anti-Iran grouplet is listed as a terrorist entity by much of the international community. Its members fled Iran in 1986 for Iraq, where it enjoyed the support of the Saddam regime.